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Monday, February 11, 2008

Antimicrobial Studies of Eucalyptus Essential Oils

Eucalyptus essential oils have been extensively studied for thier antibacterial action particularly for infections of the respiratory tract. Here are a few of the study abstracts:

Antibacterial effects of Eucalyptus globulus leaf extract on pathogenic bacteria isolated from specimens of patients with respiratory tract disorders.

Salari MH, Amine G, Shirazi MH, Hafezi R, Mohammadypour M.Department of Pathobiology, School of Public Health and Institute of Public Health Research, Tehran University of Medical Sciences, Tehran, Iran.

The antibacterial activity of Eucalyptus globulus (Blue Gum Eucaplytpus essential oil) leaf extract was determined for 56 isolates of Staphylococcus aureus, 25 isolates of Streptococcus pyogenes, 12 isolates of Streptococcus pneumoniae and seven isolates of Haemophilus influenzae obtained from 200 clinical specimens of patients with respiratory tract disorders. MIC50s for these species were 64, 32, 16 and 16 mg/L, respectively; MIC90s were 128, 64, 32 and 32 mg/L, respectively; and MBCs were 512, 128, 64 and 64 mg/L, respectively. These results suggest that further studies to clarify the possible therapeutic role of E. globulus leaf extract in the treatment of respiratory tract infection are warranted.

Effect of eucalyptus essential oil on respiratory bacteria and viruses.

Cermelli C, Fabio A, Fabio G, Quaglio P.Dipartimento di Scienze di SanitÓ Pubblica, UniversitÓ di Modena e Reggio Emilia, Via Campi 287, 41100, Modena, Italy.

The activity of Eucalyptus globulus essential oil was determined for 120 isolates of Streptococcus pyogenes, 20 isolates of S. pneumoniae, 40 isolates of S. agalactiae, 20 isolates of Staphylococcus aureus, 40 isolates of Haemophilus influenzae, 30 isolates of H. parainfluenzae, 10 isolates of Klebsiella pneumoniae, 10 isolates of Stenotrophomonas maltophilia and two viruses, a strain of adenovirus and a strain of mumps virus, all obtained from clinical specimens of patients with respiratory tract infections. The cytotoxicity was evaluated on VERO cells by the MTT test. The antibacterial activity was evaluated by the Kirby Bauer paper method, minimum inhibitory concentration, and minimum bactericidal concentration. H. influenzae, parainfluenzae, and S. maltophilia were the most susceptible, followed by S. pneumoniae. The antiviral activity, assessed by means of virus yield experiments titered by the end-point dilution method for adenovirus, and by plaque reduction assay for mumps virus, disclosed only a mild activity on mumps virus.

Antibacterial, antifungal, and anticancer activities of volatile oils and extracts from stems, leaves, and flowers of Eucalyptus sideroxylon and Eucalyptus torquata.

Ashour HM.Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Egypt.

Eucalyptus species leaves have been traditionally used to heal wounds and fungal infections. Essential oils and extracts of some Eucalyptus species possess antimicrobial and antitumor properties. We sought to determine antimicrobial and cytotoxic activities of oils and extracts of leaves, stems, and flowers of Eucalyptus sideroxylon and Eucalyptus torquata grown in Egypt. An agar diffusion method was used to analyze antimicrobial activities of essential oils and extracts of Eucalyptus against medically important gram-positive and gram-negative bacteria. A sulphorhodamine B assay was used to analyze the in vitro cytotoxic activities of oils and extracts against Human hepatocellular carcinoma cell line (HEPG2), and Human breast adenocarcinoma cell line (MCF7). Gram-positive bacteria were highly susceptible to oils and extracts of both Eucalyptus species. With the exception of Escherichia coli, gramnegative bacteria were resistant to extracts, but susceptible to the oil obtained from at least one organ of E sideroxylon and E torquata. Although Aspergillus flavus and Aspergillus niger were resistant to the extracts, essential oils of E sideroxylon and E torquata generally exhibited moderate to high antifungal activities against Candida albicans, A flavus and A niger. Oils of E torquata stems exhibited cytotoxic activities on MCF7 cells followed by oils of E torquata leaves and E sideroxylon leaves. However, oils from both species failed to exert cytotoxic effects on HEPG2 cells. This is the first report of antimicrobial and antitumor properties of E sideroxylon and E torquata. Results suggest a wider use of Eucalyptus species products in pharmaceutical, cosmetic, and food preparations.








*The FDA has not evaluated the statements on this website. The information presented here is for educational purposes of traditional uses and is not intended to diagnose, treat, cure, or prevent any diseases.


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